2OMI
Structure of human insulin cocrystallized with protamine
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 60.520, 61.840, 86.000 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 29.100 - 2.240 |
| R因子 | 0.20276 |
| Rwork | 0.199 |
| R-free | 0.26838 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | insulin hexamer R-conformation |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.022 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.941 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 2.400 |
| 分解能 [Å] (高) | 2.240 | 2.240 |
| Rmerge_l_obs | 0.107 | 0.286 |
| 独立反射数 | 15881 | |
| <I/σ(I)> | 12.1 | 4.1 |
| 完全性 [%] | 98.6 | 97.6 |
| 冗長性 | 6.5 | 2.8 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 291 | 50mM resorcinol, 400mM NaCl, 1.0mg/ml protamine 30mM phosphate buffer, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
