2IVZ
Structure of TolB in complex with a peptide of the colicin E9 T- domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-12 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 130.092, 39.933, 154.848 |
Unit cell angles | 90.00, 110.62, 90.00 |
Refinement procedure
Resolution | 144.340 - 2.000 |
R-factor | 0.17 |
Rwork | 0.168 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c5k |
RMSD bond length | 0.013 |
RMSD bond angle | 1.515 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 144.000 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.140 | 0.400 |
Number of reflections | 102162 | |
<I/σ(I)> | 14.1 | 6.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.3 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 24% POLYETHYLENE GLYCOL MONOMETHYL ETHER 5000, 80 MM CACL2, 100 MM HEPES, PH 7.5 |