2I3T

Bub3 complex with Mad3 (BubR1) GLEBS motif

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	120
Detector technology	CCD
Collection date	2004-10-17
Detector	ADSC QUANTUM 315
Wavelength(s)	1.18070
Spacegroup name	P 1 21 1
格子定数 [Å]	51.352, 173.335, 90.019
格子定数 [度]	90.00, 95.30, 90.00

精密化法

残基	50.000 - 2.800
R因子	0.242
Rwork	0.238
R-free	0.26730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u4c
結合長の平均二乗偏差(RMSD) [Å]	0.008
結合角の平均二乗偏差(RMSD) [度]	1.512
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.850
分解能 [Å] (高)	2.800	2.800
Rmerge_l_obs		0.367
独立反射数	40970
<I/σ(I)>		1.9
完全性 [%]	95.2	76.1

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7.5	296	17% PEG 4K 10% isopropanol 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K