2HAZ
Crystal structure of the first fibronectin domain of human NCAM1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-25 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9766 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 42.760, 53.880, 74.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.700 |
Rwork | 0.212 |
R-free | 0.28600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | : 1UEM |
RMSD bond length | 0.010 |
RMSD bond angle | 1.286 |
Data scaling software | XSCALE |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.790 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmerge | 0.095 | 0.487 |
Number of reflections | 9851 | |
<I/σ(I)> | 12.88 | 3.1 |
Completeness [%] | 99.0 | 94.4 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 298 | 0.3 M Calcium chloride, 17% PEG 3350, 10% PEG 400 (the latter only in the drop), pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |