2H1X
Crystal structure of 5-hydroxyisourate Hydrolase (formerly known as TRP, Transthyretin Related Protein)
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.91 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 58.078, 64.135, 58.937 |
| 格子定数 [度] | 90.00, 94.00, 90.00 |
精密化法
| 残基 | 58.820 - 1.980 |
| R因子 | 0.20809 |
| Rwork | 0.206 |
| R-free | 0.25168 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f41 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.159 |
| Data reduction software | MOSFLM |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 500.000 | 2.090 |
| 分解能 [Å] (高) | 1.980 | 1.980 |
| Rmerge_l_obs | 0.048 | 0.190 |
| 独立反射数 | 26947 | |
| <I/σ(I)> | 9.9 | 3.5 |
| 完全性 [%] | 89.1 | 89.1 |
| 冗長性 | 3.2 | 2.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1 M HEPES, 20% PEG 10000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
