2GUU
crystal structure of Plasmodium vivax orotidine 5-monophosphate decarboxylase with 6-aza-UMP bound
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-04-05 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 2 |
| 格子定数 [Å] | 69.731, 82.151, 59.739 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 32.090 - 1.860 |
| R因子 | 0.1899 |
| Rwork | 0.188 |
| R-free | 0.23614 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ffc |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.015 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.456 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (V. 1.3.1) |
| Refinement software | REFMAC (5.2.0019) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 32.090 | 1.890 |
| 分解能 [Å] (高) | 1.860 | 1.860 |
| Rmerge_l_obs | 0.100 | 0.823 |
| 独立反射数 | 27267 | |
| <I/σ(I)> | 9.8 | 1.57 |
| 完全性 [%] | 91.8 | 54 |
| 冗長性 | 7 | 5.1 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION | 8 | 297 | 30% PEG 4K, 0.2 M Sodium Acetate, 0.1M Tris HCl, pH 8.0, VAPOR DIFFUSION, temperature 297K |
