2GGA
CP4 EPSP synthase liganded with S3P and Glyphosate
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-10-17 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 63.086, 44.979, 77.251 |
| 格子定数 [度] | 90.00, 106.45, 90.00 |
精密化法
| 残基 | 15.000 - 1.700 |
| R因子 | 0.158 |
| Rwork | 0.158 |
| R-free | 0.18400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gg6 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.010 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.630 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 15.000 | 1.740 |
| 分解能 [Å] (高) | 1.700 | 1.700 |
| Rmerge_l_obs | 0.070 | 0.377 |
| 独立反射数 | 44653 | |
| <I/σ(I)> | 29.3 | 4.6 |
| 完全性 [%] | 99.3 | 97.3 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | (NH4)2SO4 / KCl / PEG 400 / HEPES-Na , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
