2G8G
Structurally mapping the diverse phenotype of Adeno-Associated Virus serotype 4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.916 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 339.600, 319.200, 285.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.200 |
Rwork | 0.263 |
R-free | 0.27300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fpv |
RMSD bond length | 0.008 |
RMSD bond angle | 1.601 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | GLRF |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.200 |
Number of reflections | 163565 |
<I/σ(I)> | 4.41 |
Completeness [%] | 75.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | virus concentrations ranging from 2 to 4 mg/ml in 20 mM Tris HCl, pH 7.5, with 2 mM MgCl, 150 mM NaCl and 1 1.5% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |