2FVY
High Resolution Glucose Bound Crystal Structure of GGBP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-12 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.83 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 119.989, 36.238, 80.101 |
Unit cell angles | 90.00, 124.50, 90.00 |
Refinement procedure
Resolution | 9.000 - 0.920 |
R-factor | 0.1179 |
Rwork | 0.110 |
R-free | 0.12960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Salmonella Typhimuruim GGBP |
RMSD bond length | 0.018 |
RMSD bond angle | 0.032 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 9.000 | 0.950 |
High resolution limit [Å] | 0.920 | 0.920 |
Rmerge | 0.045 | 0.247 |
Number of reflections | 187424 | |
<I/σ(I)> | 36.8 | 2.7 |
Completeness [%] | 95.3 | 74.7 |
Redundancy | 4.2 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 25% PEG 4000, 5% Glycerol, 0.1M HEPES PH 7.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |