2FNE
The crystal structure of the 13th PDZ domain of MPDZ
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 47.943, 62.380, 53.128 |
| 格子定数 [度] | 90.00, 106.26, 90.00 |
精密化法
| 残基 | 39.500 - 1.830 |
| R因子 | 0.19415 |
| Rwork | 0.190 |
| R-free | 0.24293 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2byg |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.015 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.556 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 39.500 | 1.950 |
| 分解能 [Å] (高) | 1.830 | 1.830 |
| Rmerge_l_obs | 0.051 | 0.475 |
| 独立反射数 | 26605 | |
| <I/σ(I)> | 2.15 | |
| 完全性 [%] | 99.9 | 100 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 8% PEG P3350, 0.1M acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
