2EWF
Crystal structure of the site-specific DNA nickase N.BspD6I
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-25 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9198, 0.9200, 1.05 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.553, 92.562, 76.349 |
Unit cell angles | 90.00, 111.88, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.840 |
R-factor | 0.2 |
Rwork | 0.183 |
R-free | 0.23600 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.022 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.870 |
High resolution limit [Å] | 1.840 | 1.840 |
Rmerge | 0.080 | |
Number of reflections | 67523 | |
<I/σ(I)> | 12.6 | |
Completeness [%] | 99.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 298 | 0.04 M KH2PO4, 16% (w/v) PEG 8000, 20% (v/v) glycerol, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |