2EQ7
Crystal structure of lipoamide dehydrogenase from thermus thermophilus HB8 with psbdo
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2006-07-15 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
格子定数 [Å] | 85.028, 107.073, 131.706 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 39.010 - 1.800 |
R因子 | 0.207 |
Rwork | 0.207 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2eq6 |
結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
結合角の平均二乗偏差(RMSD) [度] | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 50.000 | 1.860 |
分解能 [Å] (高) | 1.800 | 1.800 |
Rmerge_l_obs | 0.075 | 0.413 |
独立反射数 | 111571 | |
<I/σ(I)> | 25.6 | 3.6 |
完全性 [%] | 99.8 | 99.6 |
冗長性 | 7.1 | 6.6 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 45%(v/v) MPD, 200mM NaCl, 10mM NAD+, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |