2DPY
Crystal structure of the flagellar type III ATPase FliI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-15 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.160, 72.750, 125.740 |
Unit cell angles | 90.00, 94.13, 90.00 |
Refinement procedure
Resolution | 45.980 - 2.400 |
R-factor | 0.244 |
Rwork | 0.244 |
R-free | 0.29500 |
Structure solution method | MIR |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.130 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.068 | 0.238 |
Number of reflections | 33960 | |
<I/σ(I)> | 4.9 | |
Completeness [%] | 99.6 | 98.8 |
Redundancy | 3.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 8% PEG 8000, 0.1M calcium acetate, 3% MPD, 0.1M imidazole, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |