2C53
A comparative study of uracil DNA glycosylases from human and herpes simplex virus type 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.406, 61.161, 43.620 |
Unit cell angles | 90.00, 93.15, 90.00 |
Refinement procedure
Resolution | 14.880 - 1.800 |
R-factor | 0.164 |
Rwork | 0.164 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.700 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.080 | 0.260 |
Number of reflections | 19411 | |
<I/σ(I)> | 13.83 | 2.27 |
Completeness [%] | 93.7 | 63.1 |
Redundancy | 3.3 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |