2C4G

STRUCTURE OF CDK2-CYCLIN A WITH PHA-533514

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-20
Detector	ADSC CCD
Spacegroup name	P 62 2 2
格子定数 [Å]	191.985, 191.985, 205.880
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	29.930 - 2.700
Rwork	0.233
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fin
結合長の平均二乗偏差(RMSD) [Å]	0.007
結合角の平均二乗偏差(RMSD) [度]	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNX (2002)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	30.000	2.800
分解能 [Å] (高)	2.700	2.700
Rmerge_l_obs	0.080	0.700
独立反射数	61565
<I/σ(I)>	17	2.4
完全性 [%]	99.7	99.4
冗長性	5.2	5.1

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1		7		20% AMMONIUM SULPHATE, 1M KCL, 40MM HEPES PH 7