2BX2
Catalytic domain of E. coli RNase E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 195.838, 195.838, 143.569 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 2.850 |
R-factor | 0.231 |
Rwork | 0.230 |
R-free | 0.25700 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.551 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.850 |
Rmerge | 0.100 | 0.810 |
Number of reflections | 36133 | |
<I/σ(I)> | 17.9 | 3.5 |
Completeness [%] | 97.4 | 96.7 |
Redundancy | 9.2 | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | CRYSTALS OF THE RNASE E CATALYTIC DOMAIN/ RNA COMPLEX APPEARED AFTER TWO TO FOUR WEEKS IN 5 TO 20 % WT/V POLYETHYLENE GLYCOL 8,000, 0.1 M TRIS PH 7.5 TO 8.0, AND 10 TO 50 MM MAGNESIUM FORMATE AT 20OC |