2BTV

ATOMIC MODEL FOR BLUETONGUE VIRUS (BTV) CORE

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	281
Detector technology	IMAGE PLATE
Detector	MAR scanner 300 mm plate
Spacegroup name	P 21 21 2
格子定数 [Å]	795.600, 821.800, 753.300
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	100.000 - 3.500
R因子	0.266
Rwork	0.266
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.007
結合角の平均二乗偏差(RMSD) [度]	24.430 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	100.000	4.000
分解能 [Å] (高)	3.500	3.500
Rmerge_l_obs	0.229	0.677 *
Total number of observations	3299866 *
独立反射数	21510811 *
<I/σ(I)>	2.9	0.72
完全性 [%]	53.5 *	78 *
冗長性	6.5	1.06

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Vapor diffusion, sitting drop *	8		Burroughs, J.N., (1995) Virology, 210, 217. *

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	reservoir	ammonium sulfate	11-16 (%sat)
2	1	reservoir	Tris-HCl	0.1 (M)
3	1	reservoir	ethylene glycol	15 (%)
4	1	drop	protein	3 (mg/ml)