2AZO
DNA MISMATCH REPAIR PROTEIN MUTH FROM E. COLI
Experimental procedure
Temperature [K] | 98 |
Detector technology | IMAGE PLATE |
Collection date | 1997-04 |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.860, 111.570, 54.930 |
Unit cell angles | 90.00, 96.53, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.225 * |
Rwork | 0.202 |
R-free | 0.28900 * |
Structure solution method | SIRAS |
RMSD bond length | 0.006 * |
RMSD bond angle | 1.399 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.370 |
High resolution limit [Å] | 2.300 | 2.280 |
Rmerge | 0.079 | 0.369 |
Number of reflections | 20757 | |
<I/σ(I)> | 10 | 2 |
Completeness [%] | 95.2 | 83.8 |
Redundancy | 2.6 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.8 * | 20 * | 100MM AMMONIUM ACETATE, 50 MM MAGNESIUM ACETATE, 1 MM DTT AND 12-16 % PEG6000, pH 6.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium acetate | 100 (mM) | |
2 | 1 | reservoir | magnesium acetate | 50 (mM) | |
3 | 1 | reservoir | dithiothreitol | 1 (mM) | |
4 | 1 | reservoir | PEG6000 | 12-16 (%) |