2A65
Crystal structure of LEUTAA, a bacterial homolog of Na+/Cl--dependent neurotransmitter transporters
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9950 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 87.860, 86.310, 81.020 |
Unit cell angles | 90.00, 95.73, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.650 |
Rwork | 0.199 |
R-free | 0.21700 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.110 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Number of reflections | 70306 | |
<I/σ(I)> | 30.5 | 1.81 |
Completeness [%] | 97.3 | 85.9 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | PEGMME550, NaCl, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | PEGMME550, NaCl, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | PEGMME550, NaCl, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |