2A65
Crystal structure of LEUTAA, a bacterial homolog of Na+/Cl--dependent neurotransmitter transporters
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9950 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 87.860, 86.310, 81.020 |
| 格子定数 [度] | 90.00, 95.73, 90.00 |
精密化法
| 残基 | 50.000 - 1.650 |
| Rwork | 0.199 |
| R-free | 0.21700 |
| Structure solution method | MAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.110 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 1.710 |
| 分解能 [Å] (高) | 1.650 | 1.650 |
| 独立反射数 | 70306 | |
| <I/σ(I)> | 30.5 | 1.81 |
| 完全性 [%] | 97.3 | 85.9 |
| 冗長性 | 4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | PEGMME550, NaCl, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | PEGMME550, NaCl, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | PEGMME550, NaCl, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
