2ZZV
Crystal Structure of a Periplasmic Substrate Binding Protein in Complex with Calcium and Lactate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Detector technology | IMAGE PLATE |
Collection date | 2006-07-09 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.052, 77.335, 136.389 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.330 - 1.400 |
R-factor | 0.165 |
Rwork | 0.165 |
R-free | 0.18500 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Number of reflections | 147549 | |
<I/σ(I)> | 32.8 | 4.1 |
Completeness [%] | 93.7 | 88.3 |
Redundancy | 5.2 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 4.5% 2-propanol, 0.086M sodium citrate (pH5.5), 18% PEG 4000, 4.5% glycerol, 0.9% n-octyl-beta-D-glucopyranoside, VAPOR DIFFUSION, SITTING DROP, temperature 293K |