2ZZR
Crystal structure of unsaturated glucuronyl hydrolase from Streptcoccus agalactiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Detector technology | CCD |
| Collection date | 2007-04-15 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.7 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 116.080, 116.080, 78.125 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.000 - 1.750 |
| R-factor | 0.18308 |
| Rwork | 0.182 |
| R-free | 0.20449 |
| Structure solution method | MIR |
| Starting model (for MR) | 1vd5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.001 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.056 | 0.380 |
| Number of reflections | 61457 | |
| <I/σ(I)> | 33.2 | 3.89 |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |
