2ZZM
The complex structure of aTrm5 and tRNALeu
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 78.399, 138.390, 61.654 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.030 - 2.650 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yx1 and 1wz2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.650 |
| Number of reflections | 20282 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop, vapor diffusion | 7.2 | 293 | 175mM Mg(HCOO)2, 13% PEG 3350, 25mM KCl, 35mM Na-HEPES, pH 7.2, sitting drop, vapor diffusion, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Mg(HCOO)2 | ||
| 10 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | PEG 3350 | ||
| 3 | 1 | 1 | KCl | ||
| 4 | 1 | 1 | Na-HEPES | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | Mg(HCOO)2 | ||
| 7 | 1 | 2 | PEG 3350 | ||
| 8 | 1 | 2 | KCl | ||
| 9 | 1 | 2 | Na-HEPES |
