2ZZL
Structure of bacteriorhodopsin's M intermediate at pH 7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-22 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.912 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 102.210, 102.210, 112.480 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.030 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iw9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.176 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.200 | 2.430 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.084 | 0.521 |
| Number of reflections | 21621 | |
| <I/σ(I)> | 22.8 | 4.5 |
| Completeness [%] | 93.7 | 71.4 |
| Redundancy | 14.4 | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Membrane fusion method | 5.2 | 293 | 2.2M ammonium sulfate, 0.1M sodium citrate, 0.16M potasium chloride, 3M ammonium sulfate, 0.1M HEPES pH 7, liquid propane, Membrane fusion method, temperature 293K |
