2ZYP
X-ray structure of hen egg-white lysozyme with poly(allyl amine)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-20 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 76.940, 76.940, 37.331 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.660 - 1.180 |
| R-factor | 0.20409 |
| Rwork | 0.203 |
| R-free | 0.22368 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lkr |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.998 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.660 | 1.200 |
| High resolution limit [Å] | 1.180 | 1.180 |
| Rmerge | 0.061 | 0.109 |
| Number of reflections | 68455 | |
| Completeness [%] | 95.1 | 82.3 |
| Redundancy | 4.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1% PAA, 1.0M sodium chloride, 0.1M sodium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
