2ZYM
Crystal structure of cyclo/maltodextrin-binding protein complexed with alpha-cyclodextrin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 83.236, 46.274, 85.647 |
Unit cell angles | 90.00, 94.26, 90.00 |
Refinement procedure
Resolution | 40.420 - 1.800 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zyk |
RMSD bond length | 0.006 |
RMSD bond angle | 1.000 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.040 | 0.245 |
Number of reflections | 28257 | |
<I/σ(I)> | 34.6 | 6.5 |
Completeness [%] | 91.9 | 83.6 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 20% PEG6000, 50mM MES, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |