2ZYC

Crystal structure of peptidoglycan hydrolase from Sphingomonas sp. A1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-20
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.0000
Spacegroup name	P 43 21 2
Unit cell lengths	54.280, 54.280, 102.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.970 - 1.740
R-factor	0.201
Rwork	0.199
R-free	0.23777
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.019
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXDE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.800
High resolution limit [Å]	1.740	1.740
Rmerge	0.055	0.396
Number of reflections	16510
<I/σ(I)>	14.6	4.28
Completeness [%]	100.0	99.9
Redundancy	10.8	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	1.0M Ammonium phosphate, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	1.0M Ammonium phosphate, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K