2ZY6
Crystal structure of a truncated tRNA, TPHE39A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.70 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 62.366, 62.366, 115.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.420 - 1.750 |
| R-factor | 0.2086 |
| Rwork | 0.207 |
| R-free | 0.24037 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.213 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 13064 | |
| <I/σ(I)> | 23.9 | 12.7 |
| Completeness [%] | 94.1 | 90.3 |
| Redundancy | 15.3 | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 40% MPD, 0.1M HEPES, 0.2M Sodium Chloride, 0.2M Calcium acetate, pH7.00, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 10 | 1 | 2 | HOH | ||
| 2 | 1 | 1 | HEPES | ||
| 3 | 1 | 1 | Sodium Chloride | ||
| 4 | 1 | 1 | Calcium acetate | ||
| 5 | 1 | 1 | HOH | ||
| 6 | 1 | 2 | MPD | ||
| 7 | 1 | 2 | HEPES | ||
| 8 | 1 | 2 | Sodium Chloride | ||
| 9 | 1 | 2 | Calcium acetate |
