2ZXY
Crystal Structure of Cytochrome c555 from Aquifex aeolicus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-27 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.7 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 48.529, 32.993, 49.668 |
Unit cell angles | 90.00, 115.05, 90.00 |
Refinement procedure
Resolution | 15.920 - 1.150 |
R-factor | 0.12899 |
Rwork | 0.127 |
R-free | 0.16386 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 0.930 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.190 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmerge | 0.047 | |
Number of reflections | 25402 | |
<I/σ(I)> | 10.01 | |
Completeness [%] | 97.0 | 89.4 |
Redundancy | 3.71 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 39% PEG 4000, 0.8M ammonium acetate, 0.1M tri-sodium citrate dehydrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 39% PEG 4000, 0.8M ammonium acetate, 0.1M tri-sodium citrate dehydrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |