2ZXR

Crystal structure of RecJ in complex with Mg2+ from Thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2008-07-18
Detector	RIGAKU RAXIS V
Wavelength(s)	1.000000
Spacegroup name	P 43 21 2
Unit cell lengths	83.357, 83.357, 251.056
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.010 - 2.150
Rwork	0.235
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zxo
RMSD bond length	0.011
RMSD bond angle	1.700
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MLPHARE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.150	2.150
Rmerge	0.064	0.276
Number of reflections	48684
<I/σ(I)>	47.2	3.3
Completeness [%]	98.5	86
Redundancy	17.2	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.05M ADA, 6% PEG4000, 1% 2-propanol. The crystal was soaked in cryoprotecting solution containg 20% glycerol and 10mM magnesium sulfate, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K