2ZXO

Crystal structure of RecJ from Thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2008-05-19
Detector	RIGAKU RAXIS V
Wavelength(s)	1.000000
Spacegroup name	P 43 21 2
Unit cell lengths	82.984, 82.984, 249.223
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.720 - 2.500
Rwork	0.230
R-free	0.28200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ir6
RMSD bond length	0.011
RMSD bond angle	1.700
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MLPHARE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.070	0.302
Number of reflections	31284
<I/σ(I)>	45.4	6.2
Completeness [%]	100.0	99.9
Redundancy	13.1	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.05M ADA, 6% PEG4000, 1% 2-propanol. The crystal was soaked in cryoprotecting solution containg 20% glycerol, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K