2ZXK
Crystal structure of SeMet-Red chlorophyll catabolite reductase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Detector technology | CCD |
| Collection date | 2008-07-19 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97912, 0.97940, 0.96406, 0.99500 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.978, 83.539, 120.404 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.480 - 2.500 |
| R-factor | 0.21929 |
| Rwork | 0.215 |
| R-free | 0.29085 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.753 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.085 | 0.328 |
| Number of reflections | 19469 | |
| <I/σ(I)> | 11.6 | 8.5 |
| Completeness [%] | 98.8 | 91.6 |
| Redundancy | 11.9 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 22% PEG 4000, 0.1M ammonium sulfate, 0.1M sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
