2ZXD
alpha-L-fucosidase complexed with inhibitor, iso-6FNJ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 181.012, 181.012, 170.322 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.150 |
| R-factor | 0.1636 |
| Rwork | 0.162 |
| R-free | 0.20270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB enrty 2zwz |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.747 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.070 | 0.547 |
| Number of reflections | 58320 | |
| <I/σ(I)> | 37.5 | 4.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.3 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 298 | 14% PEG 5000 MME, 0.1M Tris-HCl, pH 7.8, 5% Jeffamine M-600, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
