2ZXB
alpha-L-fucosidase complexed with inhibitor, ph-6FNJ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 179.517, 179.517, 169.098 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.610 |
| R-factor | 0.1756 |
| Rwork | 0.172 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB enrty 2zwz |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.992 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.700 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Rmerge | 0.068 | 0.511 |
| Number of reflections | 31842 | |
| <I/σ(I)> | 23.5 | 3.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 298 | 15% PEG 5000 MME, 0.1M Tris-HCl, pH 8.2, 5% Jeffamine M-600, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
