2ZX5
alpha-L-fucosidase complexed with inhibitor, F10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-14 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 180.520, 180.520, 169.266 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.650 |
| R-factor | 0.1941 |
| Rwork | 0.191 |
| R-free | 0.25620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB enrty 2ZWZ |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.906 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.125 | 0.501 |
| Number of reflections | 30844 | |
| <I/σ(I)> | 10.3 | 3.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.19 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 298 | 15% PEG 5000 MME, 0.1M HEPES, pH 7.2, 5% Jeffamine M-600, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
