2ZWU
Crystal Structure of Camphor Soaked Ferric Cytochrome P450cam
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-22 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.7 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 63.608, 63.608, 250.389 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.900 - 1.300 |
R-factor | 0.16644 |
Rwork | 0.166 |
R-free | 0.18386 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cpp |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP (9.4.09) |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.900 | 1.350 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.075 | 0.407 |
Number of reflections | 125182 | |
<I/σ(I)> | 34.4 | 4.9 |
Completeness [%] | 98.6 | 98.9 |
Redundancy | 4.9 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298 | 11-14% PEG 4000, 50mM Tris-HCl, 250mM KCl, 1mM d-camphor, 10mM dithioerythritol(DTE); After crystallization, soaked camphor saturated buffer for 2 days, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |