2ZWR

Crystal structure of TTHA1623 from thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-10
Detector	RIGAKU JUPITER 210
Spacegroup name	I 2 2 2
Unit cell lengths	79.136, 114.122, 114.711
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.460 - 2.200
R-factor	0.18982
Rwork	0.188
R-free	0.22896
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.225
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Number of reflections	26556
Completeness [%]	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	293	100mM sodium cacodylate, 30% PEG200, 200mM zinc acetate, PH7.3, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K
1	VAPOR DIFFUSION, SITTING DROP	7.3	293	100mM sodium cacodylate, 30% PEG200, 200mM zinc acetate, PH7.3, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K