2ZWI
Crystal structure of alpha/beta-Galactoside alpha-2,3-Sialyltransferase from a Luminous Marine Bacterium, Photobacterium phosphoreum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.429, 57.827, 59.246 |
| Unit cell angles | 90.06, 106.76, 103.20 |
Refinement procedure
| Resolution | 20.690 - 2.010 |
| R-factor | 0.17574 |
| Rwork | 0.173 |
| R-free | 0.22834 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z4t |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.204 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 43495 |
| <I/σ(I)> | 13.804 |
| Completeness [%] | 97.8 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 22% PEG3350, 0.2M KCl, 2mM ZnSO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
