2ZVY
Structure of the periplasmic domain of MotB from Salmonella (crystal form II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 35 |
| Detector technology | CCD |
| Collection date | 2008-02-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 64.250, 86.400, 129.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.970 - 1.750 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zov |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.970 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 36833 | |
| <I/σ(I)> | 15.1 | 4.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 30% PEG 4000, 0.1M Tris-HCl, 0.2M Sodium Acetate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
