2ZVR
Crystal structure of a D-tagatose 3-epimerase-related protein from Thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-09-03 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.245, 55.211, 58.714 |
| Unit cell angles | 107.14, 102.71, 91.54 |
Refinement procedure
| Resolution | 37.600 - 2.200 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.24300 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.630 | 2.250 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Number of reflections | 29673 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 20-30% PEG 200, 5% PEG 1000, 0.1M MES, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
