2ZVL
Crystal structure of PCNA in complex with DNA polymerase kappa fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-03 |
| Detector | RIGAKU MSC JUPITER 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 74.769, 74.707, 109.049 |
| Unit cell angles | 87.25, 77.46, 80.26 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.22865 |
| Rwork | 0.225 |
| R-free | 0.28943 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vym |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.872 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.082 | 0.241 |
| Number of reflections | 71006 | |
| <I/σ(I)> | 9.1 | 4.1 |
| Completeness [%] | 90.6 | 90.6 |
| Redundancy | 2.7 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | HANGING DROP VAPOR DIFFUSION | 5.2 | 293 | pH5.2, HANGING DROP VAPOR DIFFUSION, temperature 293K |
