2ZVF
Crystal structure of Archaeoglobus fulgidus alanyl-tRNA synthetase C-terminal dimerization domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 124.109, 131.714, 138.823 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.200 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.27600 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.780 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.105 | 0.386 |
| Number of reflections | 38548 | |
| <I/σ(I)> | 18.4 | 2.18 |
| Completeness [%] | 98.7 | 91.3 |
| Redundancy | 9.7 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 1.6M ammonium sulfate, 7.5% hexanediol, pH 8.0, VAPOR DIFFUSION, temperature 293K |
