2ZV3
Crystal structure of project MJ0051 from Methanocaldococcus jannaschii DSM 2661
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 149.850, 70.790, 96.010 |
| Unit cell angles | 90.00, 121.67, 90.00 |
Refinement procedure
| Resolution | 40.920 - 2.100 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wn2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 0.358 |
| Number of reflections | 49935 | |
| <I/σ(I)> | 8.8 | 3.4 |
| Completeness [%] | 99.6 | 97.8 |
| Redundancy | 4.8 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Oil microbach | 4.6 | 295 | 30w/v(%) PEG 4000, 0.1M Acetate, 0.2M Ammonium Acetate, pH 4.6, Oil microbach, temperature 295K |
