2ZUY
Crystal structure of exotype rhamnogalacturonan lyase YesX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-20 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.853, 88.070, 99.304 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.190 - 1.650 |
| R-factor | 0.16177 |
| Rwork | 0.161 |
| R-free | 0.18425 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z8r |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.104 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.094 | 0.258 |
| Number of reflections | 77151 | |
| <I/σ(I)> | 8.2 | 4.1 |
| Completeness [%] | 98.9 | 97.4 |
| Redundancy | 9.8 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 293 | 20% PEG 3350, 0.2M Ammonium acetate, pH7.1, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
