2ZUX
Crystal structure of rhamnogalacturonan lyase YesW complexed with rhamnose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-20 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.8000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.282, 105.858, 100.967 |
| Unit cell angles | 90.00, 94.82, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.320 |
| R-factor | 0.16704 |
| Rwork | 0.166 |
| R-free | 0.18033 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z8r |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.166 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.370 |
| High resolution limit [Å] | 1.320 | 1.320 |
| Rmerge | 0.066 | 0.338 |
| Number of reflections | 280440 | |
| <I/σ(I)> | 13 | 2.8 |
| Completeness [%] | 97.6 | 95.1 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 293 | 55% MPD, 0.1M TRIS, pH8.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
