2ZUU

Crystal structure of Galacto-N-biose/Lacto-N-biose I phosphorylase in complex with GlcNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2007-01-28
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 1
Unit cell lengths	67.777, 111.484, 118.432
Unit cell angles	105.19, 90.48, 107.35

Refinement procedure

Resolution	40.680 - 2.300
R-factor	0.173
Rwork	0.170
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.218
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.054	0.246
Number of reflections	137195
<I/σ(I)>	15.293	2.5
Completeness [%]	97.8	97.5
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	277	Na cacodyrate, Mg(NO3)2, PEG 4000, GlcNAc, pH 6.5, VAPOR DIFFUSION, temperature 277K
1	VAPOR DIFFUSION	6.5	277	Na cacodyrate, Mg(NO3)2, PEG 4000, GlcNAc, pH 6.5, VAPOR DIFFUSION, temperature 277K