2ZUF
Crystal structure of Pyrococcus horikoshii arginyl-tRNA synthetase complexed with tRNA(Arg)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.210, 60.140, 110.330 |
| Unit cell angles | 90.00, 107.06, 90.00 |
Refinement procedure
| Resolution | 39.650 - 2.300 |
| R-factor | 0.2032 |
| Rwork | 0.201 |
| R-free | 0.26170 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2zue |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.180 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.064 | 0.210 |
| Number of reflections | 38821 | |
| <I/σ(I)> | 17.2 | 2.5 |
| Completeness [%] | 90.7 | 79.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 30% PEG 4000, 0.2M ammonium sulfate, 5mM magnesium chloride, 100mM Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 10 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | ammonium sulfate | ||
| 3 | 1 | 1 | magnesium chloride | ||
| 4 | 1 | 1 | Tris-HCl | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | PEG 4000 | ||
| 7 | 1 | 2 | ammonium sulfate | ||
| 8 | 1 | 2 | magnesium chloride | ||
| 9 | 1 | 2 | Tris-HCl |
