2ZU9
Crystal structure of mannosyl-3-phosphoglycerate synthase from Pyrococcus horikoshii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2007-12-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.655, 84.867, 83.565 |
| Unit cell angles | 90.00, 102.00, 90.00 |
Refinement procedure
| Resolution | 42.910 - 2.000 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zu7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 49826 | |
| <I/σ(I)> | 13.3 | 4.8 |
| Completeness [%] | 99.0 | 96.7 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | 30% MPD, 0.05M sodium acetate, 0.1mM GDP-mannose, 0.1mM 3-phosphoglycerate, 0.1mM CoCl2, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
