2ZU8
Crystal structure of mannosyl-3-phosphoglycerate synthase from Pyrococcus horikoshii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2007-12-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.238, 84.993, 83.592 |
| Unit cell angles | 90.00, 101.91, 90.00 |
Refinement procedure
| Resolution | 42.720 - 2.400 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zu7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 27663 | |
| <I/σ(I)> | 12.6 | 7.5 |
| Completeness [%] | 95.7 | 81.5 |
| Redundancy | 3.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | 30% MPD, 0.05M sodium acetate, 0.1mM GDP-mannose, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
