2ZTN

Hepatitis E virus ORF2 (Genotype 3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-26
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	336.864, 349.423, 359.595
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.560
Rwork	0.305
R-free	0.30900
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.680
High resolution limit [Å]	3.560	3.560
Rmerge	0.131	0.498
Number of reflections	503674
<I/σ(I)>		3.2
Completeness [%]	99.9	99.8
Redundancy	5.6	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8	293	5% PEG10000, 100mM Tris, pH8.0, EVAPORATION, temperature 293K